![]() ![]() This research provides an expanded knowledge base of genome sizes, ploidy, and reproductive pathways for diverse species and hybrids to enhance future breeding, improvement, and the genomic study of blueberry. Further karyotyping of these individuals is necessary to ascertain aneuploidy anomalies. We speculate some of these progeny to be near tetraploids with extra chromosomes from hexaploid progenitors. Interspecific pentaploid and aneuploid progeny 2C DNA content ranged from 2.61 pg to 3.15 pg. Tetraploid hybrid pedigrees, which involved hexaploid crosses within three prior generations, had a 2C value range between 2.22 pg and 2.59 pg. Of the 369 unique accessions analyzed for ploidy, 259 were tetraploid, 46 were diploid, one was triploid, 51 were pentaploid or aneuploid with 2C values between tetraploid and hexaploid values, and 12 were hexaploid. virgatum ‘Premier’ and NC4790), respectively. crassifolium ‘Well's Delight’ to 1.41 pg in V. The mean (range) DNA content of diploid, tetraploid, and hexaploid reference species were 1.20 pg (0.99 pg in V. The nuclear DNA content was analyzed via flow cytometry. A total of 369 accessions, including a diversity panel (DP) of 251 inter- and intra-specific hybrid Vaccinium accessions, as well as 118 non-hybrid Vaccinium species across multiple sections, were sampled from the North Carolina State University blueberry breeding program and the National Clonal Germplasm Repository. The objective of this study was to use flow cytometry, k-mer distribution analysis, and known pedigree information to evaluate genome sizes (2C nuclear and 1Cx monoploid), and ploidy of diverse accessions from Vaccinium sections and species. However, these approaches are complicated by variation in ploidy, triploid blocks and infertility, production of unreduced gametes, and aneuploidy. virgatum) cultivars often include introgressing regionally adapted species into the cultivated gene pools through interspecific hybridization. This release can be used with CLC Genomics Server 5.5.X.Breeding strategies for improving blueberry ( Vaccinium corymbosum and V.This release is based on CLC Assembly Cell 4.2.1 CLC Genomics Workbench Octowebsite maker Comprehensive suite of tools for analysis of next-gen sequencing data including resequencing data, workflow management, read mapping, de novo assembly, variant detection, RNA-Seq, ChIP-Seq, and trio analysis.Fixed a bug in the Assemble Sequences tool causing some data sets to produce inferior contigs.Fixed a read mapper error occurring under special circumstances when excluding regions of a reference when mapping reads.Fixed an error when importing BAM files, including problems regarding download of reference sequences. ![]() Fixed error in importing SOLiD XSQ files.Fixed crash of detailed mapping report tool with certain data sets.Fixed problem that caused a crash with extract consensus sequence tool with certain parameter configurations and with read mappings with no reads.Fixed various stability and performance problems of Maximum likelihood phylogeny.Improved stability of Probabilistic variant detection on huge data sets.Previously, they were treated as unique reads. In BAM files created by BWA, non-specific reads are now recognized as such during import.If you are using the tool with annotations spanning across the starting point of a circular reference, we recommend re-running the analysis. Fixed: The Target Regions Statistics tool did not handle annotations covering the starting point of circular reference sequences properly.Fixed: Opening a search view for searching sequences at NCBI would sometimes fail.Fixed various problems related to launching the Workbench through Java Webstart.Any existing settings will be copied to the new location automatically. The folder used by the Workbench for storing log and settings files on Windows 8 has been updated to follow conventions for Windows 8 which is identical to Windows 7.Fixed a problem in track lists that caused the view to crash when there is an insertion at the very end of a chromosome.Fixed problem of unresponsive dialogs when running analysis with many reference sequences.Powered by cutting-edge technology and accelerated. All the tools you need, integrated into a single user friendly and scalable application, and ready to generate results you can trust. Fixed: track lists would sometimes be rendered empty when scrolling tracks with insertions. CLC Genomics Workbench (CLC Gx) is a powerful solution developed by scientists for scientists to analyze and visualize next generation sequencing (NGS) data. Please see the release notes for CLC Genomics Workbench 23.0, below, for a full list of changes since the last general release of this software.Fixed: Error message when running analysis on experiments (statistical tests, clustering etc.). ![]()
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